Chemical composition and antimicrobial activity of the. Further the clove essential oil was analysed for its chemical components by gc ms. Mar 23, 2007 the essential oil extracted from the dried flower buds of clove, eugenia caryophyllata l. The chemical composition and biological activity of clove essential oil, eugenia caryophyllata syzygium aromaticum l. Chemical composition and antimicrobial activity of the essential oil of syzygium aromaticum flower bud clove against fish systemic bacteria isolated from aquaculture sites. Chemical composition, antibacterial and antioxidant.
In this technology silica gel 2002002 mm plates merk was used. Chemical composition of the essential oil obtained from the leaves of syzygium caryophyllatum has been analyzed by gc and gcms twenty four compounds representing 89. Syzygium aromaticum, brassica compestris and cow ghee. Pdf chemical composition and content of essential oil from the. The composition and antifungal activity of clove essential oil eo, obtained from syzygium aromaticum, were studied. Herbal medicinal products have been documented as a significant source for discovering new pharmaceutical molecules that have been used to treat serious diseases. Origin of clove oil a native of indonesia and the malacca islands, it is an evergreen tree that grows to about 10 meters 30 feet tall and has bright green leaves and nailshaped rosepeach flower buds which turn, upon drying, a. The essential oil, obtained by hydrodistillation of fruits, was analyzed by gc and gc ms. Chemical components of the essential oil from the buds and leaves of other species and variant of syzygium had been reported by several workers15 17.
Eugenia caryopyllata is one of the plant that have secondary metabolites that are widely used in the world of health 1. The essential oil obtained by hydrodistillation from seeds of syzygium aromaticum myrtaceae growing in benin was analyzed by gas chromatography gc and gas chromatographymass spectrometry gc ms. Nonetheless, the extraction method depends, among other factors, on the kind of material to. Effects of phenological stages on yield and composition of. A total of 26 components were identified accounting for 99. The results of these analyses are presented in table i as a relative peak area of each constituent. Europe pmc is an archive of life sciences journal literature. Chemical composition and content of essential oil from the bud of cultivated turkish clove syzygium aromaticum l. Syzygium aromaticum, essential oil, chemical composition, pectinase enzyme 1. Gcms analysis and identifications of chemical constituents. Effect of crushing of clove bud syzygium aromaticum and. The antibacterial and antioxidant activities were assessed for each oil using minimum inhibitory concentrations mics and 2,2diphenyl1picrylhydrazyl dpph methods. The chemical composition on bud, leaf and stem oil of s.
In the present study, the essential oil was extracted from the buds of eugenia caryophyllata thunb myrtaceae by hydrodistillation method and the chemical composition of essential oil was identified by gc and gc ms. Chemical composition and antimicrobial activity of. Isolation of clove syzygium aromaticum compounds thin layer chromatography was used to isolate and separate the clove syzygium aromaticum compounds. Gcms analysis of clove syzygium aromaticum bud essential. Sixteen volatile compounds were identified from the nhexane extract of the buds of syzygium aromaticum by using gas chromatographymass spectroscopy. The antibacterial activity of clove syzygium aromaticum essential oil ceo and eugenol was tested against seven gramnegative and nine grampositive fish pathogenic bacteria isolated from cultured olive flounder paralichthys olivaceus in korea. Potential of clove of syzygium aromaticum in development of a. Chemical characterization, antioxidant and antimicrobial. Phytochemical composition and pleotropic pharmacological. Gcms separation of compounds from clove essential oil syzygium aromaticum the results showed that.
Pdf gcms analysis of clove syzygium aromaticum bud. Pdf chemical constituents of clove syzygium aromaticum, f am. Antioxidant and antibacterial activities of the essential oil. A literature survey showed detailed work on chemical composition of bud and leaf essential oils of s.
The eo analysed showed a high content of eugenol 85. The essential oil of clove syzygium aromaticum obtained by hydrodistillation 0. Plants have been employed as medicine since time immemorial, and there has been a recent resurgence in the use of plants as medicines due to their little or no toxicity at the doses used for treatment of different ailments. Study of the chemical composition of essential oils by gas chromatography 309 organic solvent extraction or straight distillation as water steam has a lower cost compared to organic solvents. Chemical constituents of the essential oil from syzygium. Differences of particle size did not give significant differences in the yield of clove oil on 24 hour of distillation. Chemical composition and antioxidant activity of essential. The aim of the present study was to investigate the essential oil of.
The chemical composition and antioxidant activities of the essential oil from clove buds toward various oxidative stresses in vitro were investigated in this work. Pcpa01417j19 analysis of the composition of an essential oil, or other volatile liquid, by fast gcfid in french. Leaf essential oil composition of six syzygium species. Chemical composition and antioxidant properties of clove leaf. The chemical composition, main physicochemical properties, and nutritional value of seed flour and seed oil of lannea kerstingii were studied.
In this work, the chemical composition of essential oils of piper nigrum and syzygium aromaticum from chad were determined and their antimicrobial effect in combination with different antibiotics. Chemical composition and phytotoxic activity of clove. The antimicrobial activity of spices of human daily diet has been widely demonstrated. Journal of analytical methods in chemistry hindawi.
Clove oil was obtained commercially and analysed by gc and gc ms. The chemical constituents of clove bud oil were analyzed by using gas chromatographymass spectrometry gcms. Secondary metabolites production in clove syzygium. Chemical composition and antimicrobial activity of syzygium. Chemical composition, physicochemical characteristics, and. Antifungal activity of the clove essential oil from. Article pdf available in bioresources 22 may 2007 with 2,564 reads. The major component of clove essential oils is usually considered to be eugenol, with.
Pharmacologically, clove has been documented as the main source of phenolic molecules like hidroxibenzoic acids, flavonoids, hidroxiphenyl propens, hidroxicinamic acids, and eugenol c 10 h 12 o 2which is the major bioactive moleculeand gallic acid derivatives like hidrolizable tannins that are found in high amounts in the fresh plant. The chief constituent of clove essential oil is eugenol, which is used as a starting material for the production of vanillin 16. In vitro activity of syzygium aromaticum against food. The results showed that 22 compositions of the essential oil were identi. Syzygium aromaticum showed a lower activity on k562 ic 50 90 ppm, even though this oil did not share the same main compound with cy. Larvicidal efficacies and chemical composition of essential. Bud, leaf and stem essential oil composition of syzygium. Chemical composition and antibacterial effects of clove. Chemical composition, antibiotic promotion and in vivo.
Chemical and biological investigations of syzygium. Effect of pectinase enzyme on distillation rate of. The major component of clove oil is usually considered to be eugenol, with. This paper describes the chemical composition and antimicrobial activity of the essential oil of syzygium aromaticum against vibrio spp. International research journal of pharmacy and medical sciences issn online. Chemical composition and antioxidant activities of the oleoresin from the clove buds syzygium aromaticum xin li and jianguo xu college of food sciences, shanxi normal university, linfen, china abstract. Also, it avoids oil heating or the use of sophisticated equipment. Clove essential oil rendered the growth of all isolates of c. This plant is widely used and has commercial value because the content of secondary metabolite compounds. Study of the chemical composition of essential oils by gas. This study was conducted to identify qualitatively the chemical composition of essential oil of. In this study, clove bud oil, which was cultivated in the mediterranean region of turkey, was provided from a private essential oil company in turkey. Pharmacologically, clove has been documented as the main source of phenolic molecules like hidroxibenzoic acids, flavonoids, hidroxiphenyl propens, hidroxicinamic acids, and eugenol c 10 h 12 o 2which is the major bioactive moleculeand gallic acid derivatives like hidrolizable tannins that are found in high amounts in the fresh plant table 1 6,9,16.
Pdf chemical composition and content of essential oil from. Chemical composition of the essential oil obtained from the leaves of syzygium caryophyllatum has been analyzed by gc and gc ms twenty four compounds representing 89. Chemical composition identification of essential oil constituents was. The chemical composition and antioxidant activities of the oleoresin from clove buds were investigated in this work. Gcms analysis of clove syzygium aromaticum bud essential oil. Introduction the clove tree syzygium aromaticum called cengkeh in indonesia, was first cultivated on the island of moluccas, indonesia, in southeast asia 1. Compounds from syzygium aromaticum possessing growth. Chemical composition and in vitro cytotoxic screening of. Chemical and biological investigations of syzygium aromaticum. Many plant species have been reported to have pharmacological activities attributable to their phytoconstituents such are glycosides, saponins, flavonoids, steroids, tannins, alkaloids, terpenes, etc. The oil possesses antiemetic, analgesic, antibacterial, antifungal and anticarcinogenic properties 1015.
Chemical composition, antioxidant and the possible use as skincare ingredient of clove oil syzygium aromaticum l. The aim of this study was to analyze the chemical composition of dried clove buds volatile oil in bangladesh by gas chromatographymass spectrometry gc ms technique and also show their medicinal properties according to the characteristics of the constituents present in that essential oil. In this experiment the clove buds essential oil was extracted by hydro. Chemical composition and content of essential oil from the.
To assess the chemical composition and mosquito larvicidal potentials of essential oils of locally sourced pinus sylvestris p. Chemical composition of essential oils the analyses were successful without previous fractionation of essential oils. Chemical composition and antioxidant properties of clove. Essential oil composition of syzygium aromaticum l. Pdf chemical composition and content of essential oil. The oil yield of whole clove bud and crushed clove bud were 14. The different methods are hereby synthetically presented. Journal of essential oil research 20, 25 4, 315323. The results showed that the essential oils mainly contained about 87. Potassium was the predominant mineral, followed by magnesium and calcium. Bud, leaf and stem essential oil composition of clove. The main constituents of the essential oil are phenylpropanoids such as carvacrol, thymol. The chemical composition of clove bud oil provided from private turkish company in turkey was investigated. The chemical composition of clove essential oils was obtained by gas chromatography coupled to a mass spectrometer gc ms, according to the literature, using a qp 2010 shimadzu system equipped with an aoc20i auto injector, rtx5ms silica capillary column 30 m y 0.
Constituents were then identified by comparing the results of the chromatogram and reference retention time using wiley mass spectra library wiley w9n11. The chemical constituents of clove bud oil were analyzed by using gas chromatographymass spectrometry gc ms. Obtaining the essential oil of syzygium aromaticum. The main secondary metabolites of cloves consist of eugenol. Both oils achieved over 85% larval mortality within 24 h. The relative composition of essential oils was determined and thecompounds were identified by gc. The major components of the essential oil obtained from the buds of syzygium aromaticum were eugenol 71. The chemical composition and biological activity of clove. The gc ms analysis of clove buds eugenia caryophyllus essential oil led to identification of different compounds fig. The chemical composition and biological activity of. Gc ms separation of compounds from clove essential oil syzygium aromaticum the results showed that the essential oil mainly contained about eugenol 80. Antifungal activity of the clove essential oil from syzygium. The chemical compositions of the oil were identified by gc ms and the result as shown in table 1. Bud and leaf essential oil composition of syzygium.
This study was designed to perform simultaneous determination of three reference compounds in syzygium aromaticum sa, gallic acid, ellagic acid, and eugenol, and to investigate the chemical antagonistic effect when combining curcuma aromatica ca with sa, based on chromatographic analysis. The chemical composition of the essential oils of both plants was determined using gc ms while the larvicidal bioassay was carried out using different concentrations of the oils against the larvae of a. Potential of clove of syzygium aromaticum in development. Mics, determined according to clinical and laboratory standards institute protocols, and minimum fungicidal concentration.